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Role of Vacuum Aspirator In Cell Culture
January 30, 2024

Role of Vacuum Aspirator In Cell Culture

Role of Vacuum Aspirator In Cell Culture

Vacuum Aspirator and Cell Culture Process 

Cell culturing is one of the most critical techniques that is carried out in biological and medical laboratories. The vacuum aspirator Cell Culture enables the researcher to extract the specific cell component and dispose of the rest part of the cultural medium. So the aspiration is mainly used to isolate some particular cell types, the cell components as well as some specific cultural medium, so that not every material existing in the cell culture comes out of the medium and only the selected cell types can be extracted out for the research analysis.  

This technique plays the main role in cell lining maintenance, drug development, tissue engineering, and the study of specific cell behavior. By the usage of these cell, culture aspiration techniques, scientists can even enhance the experimental accuracies, can also enhance the cell-based evaluation, and can improve people’s understanding of cell culture. 

What is Vacuum Aspirator Cell Culture? 

The aspirator or the suction is mainly used in biological laboratories for the removal of cell culture media. Cell cultures refer to the cultivation of eukaryotic or prokaryotic cells in a specific cultural medium so that they can get enough nutrients and minerals that they need for cell proliferation. After the metabolism of culture media in the Petri dishes, these were later on sucked by the use of vacuum aspirators in the laboratories. The media is sucked by the vacuum pressure, generator by the aspirator. 

Basic Cell Culture Procedure 

  • Thawing cells: Thaw the frozen cells in a 37-degree centigrade water bath to prevent cell damage. 
  • Cell Culture: depending on the cell type and components, just mix with the culture and leave it for the required time in the incubator for cell cultivation. 
  • Addition of Lactic Acid: In the cell culture, the lactic acid addition can even leave a greater impact on the cell culture. 

The Traditional Aspiration Methods for Cell Cultures 

In the old days, the pipette was used to suck the liquid from any kind of the flask or the petri dish. The researcher mainly handled this procedure with the help of his thumb. When the researcher needed some liquid inside the sucking pipette, he tend to suck it through the mouth and later on just put the thumb at the lower end of the micropipette, so thus the liquid will remain in the tube until the required minimum quantity. However, this was one of the most damaging methods because of repetitive pressure and release movement. So thus later on the vacuum aspirators were evolved and operated by the given electrical source automatically. 

The Modern Lab Vacuum Aspirators for Cell Culture

The researchers now prefer to have self-installed vacuum aspirators. These systems are more efficient than those of the previously used pipetting system. These effectively extract the culture medium after cell metabolism and assist researchers in sucking other liquid wastes from the tissue samples. 

Aspiration System and Cell Culture Lab Essentials 

  • Cell Culture Hood: These are mainly to provide an effective aseptic area where the researcher can easily perform the cell culture procedure without any interference of microorganisms from the inside out. In addition, this aseptic area prevents the microorganisms flow out of the laminar flow hood for the effective cell culture procedure. 
  • Incubator: These are mainly intended to provide the optimum temperature for the growth and cultivation of microorganisms in the cell culture petri dish.
  • The Vacuum Aspiration System for Cell Culture: The vacuum aspiration is used for the aseptic removal of cell culture media and other reagents from the petri dish. 


Cell culture is one of the most advanced processes in the biological and medical laboratories to cultivate and proliferate the eukaryotic and prokaryotic cells by providing them with essential media to grow and necessary minerals and nutrients to get food from. However, the aspirator is used in the culturing media and to dispose of the media when it is metabolized by the living cells. The aspirator used to suck the media from the petri dish so that another medium could smear on the petri dish for another type of cell growth. The aspirator sucks the media through vacuum pressure and thus it’s an essential piece of equipment in the science laboratories. 

Other than this, aspirators are used for multiple purposes like RNA/DNA extraction, the supernatant liquid withdrawal from variable vessels inside the laboratory, and liquid waste removal in biological, medical, and even radioactive laboratories. So that whenever the researcher conducts any research, the result must be found free of any errors and mistakes. It will show accurate and precise results without any doubt. Previously, the micropipette was used to extract the unwanted liquids and fluids from the researching solids and liquid vessels but in the recent era, these vacuum aspirators are mainly used to extract materials without even disturbing the original observing sample and for the accuracy of the results and experiments in variable laboratories, particularly science and biology. 


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