Imrali Invention

How Vacuum Aspirator Work
February 5, 2024

How Vacuum Aspirator Works – A Comprehensive Guide

Table of Contents


Aspirator, a medical device, is designed for laboratory waste disposal and separation of liquid/solid. As a versatile tool, it can also be utilized in tissue culture, DNA extraction, or liquid dissociation. At Imrali, we have manufactured aspirators to meet industry standards and enable researchers and scientists to uncover truths and discoveries in a scientific discipline. 

Welcome to IMRALI INVENSTIONS aspiration system guide to three aspirators: mini, maxi, and midi. Please read the following manual carefully before using the device and consider safety procedures and operational descriptions. 

Safety Measures: 

  • As a user of the three aspirators mentioned above, one must wear suitable protective clothing to ensure the safety of the surroundings and the researcher or investigator performing experiments. 
  • Aspirators must be placed in a well-ventilated place, and experimenters, while performing relevant tasks, should choose fire-proof, dry, and non-slippery surfaces.
  • One should avoid direct contact with aspirator valves, injection pumps, and other device components during laboratory tests.
  • Ensure experienced or trained professionals operate the instrument.

Different Range of Applications: 

Aspirators can be utilized in laboratories, Research facilities, and educational institutions, which are designed to be used in the following environments:

  • No higher than 2000 meters above sea level.
  • Temperature ranges from 32 F to 104 F.
  • The device is intended for indoor installation with voltage variations of no more than ± 10% of normal levels.
  • However, the aspirator is intended to be utilized in areas other than residential areas and should be used in professional settings.

Structure of Vacuum Aspirator:

  • Vacuum Adjusting Knob:

The desired suction level can be adjusted, making it convenient for experimenters to get the desired results.

  • Vacuum Level Gauge:

Connected next to the Device knob, one can adjust its vacuum levels which allows the investigator to analyze the degree of the vacuum level. 

  • Vacuum Tube:

The suction pipe draws liquid through an aspirator pump that transfers liquid into a container. 

  • Aspiration Hand Grip:  


The instrument hand grip is for liquid intake or suction; the experimenter uses the assistance of a pipette to draw out the sample into the container.

  • Liquid and Waste Collection Container:

The primary liquid container is used for fluid collection, while the waste container is used for storing fluid waste collected during the process. 

  • Levelling Vacuum Sensors:

The vacuum pump creates a signal sound when the fluid level rises above the warning. The aspirator sensor will detect the waste fluid level. If the liquid waste level is higher, the pump will automatically stop.

Working Operation Guide to Vacuum Aspirator: 

  • The First step in operating any of the above-mentioned three aspirators is the piping installation, in which you must ensure that the pipeline is correctly connected, ensuring no outflow of liquid or fluid. 
  • You must make sure that the vacuum tube is firmly closed. 
  • The next step is to turn on the control switch on the vacuum aspirator.
  • Rotate the vacuum controller knob clockwise whilst the light is on to set the vacuum level at the desired level; it is recommended that the instrument should be handled with care and should follow necessary safety measures to perform the essential task successfully. 
  • The next step is crucial for the analysis and process in which the system should be analyzed carefully with the task requirement, so, firstly, the light on the aspirator will assist you and give a clear indication when the vacuum reaches its desired level, the light will become steady indicating you that the system has reached its full potential. Furthermore, the system will only show the level set by the investigator. Secondly, by pressing the instrument button, the vacuum aspirator allows you to contact other small containers where fluid waste is separated and collected. 
  • After completing the above process, you must shut down the vacuum aspirator system. 
  • The bleed screw in the vacuum aspirator should be released, which helps the device to close the aspirator system and facilitates the removal of unnecessary air or any other matter from the aspirator system.
  • Lastly, you must carefully follow the necessary safety procedures, remove the waste vessel, and cover. Additionally, you discard the waste in appropriate disposal sacks, ensuring the safety of the internal facility and environment.

How Vacuum Aspirator Works? 

The aspiration pump creates a vacuum in the aspiration bottle; thus, due to the differential pressure inside and outside the aspirator, the liquid usually gets pulled inside the collection bottle. Therefore, when the collection bottle gets full, the unwanted extra liquid is disposed of from the jar. In addition, before applying pressure, the vacuum level must be adjusted up to the mark on the indicator so that the specific pressure can be used and pulled out of the laboratory vessel. 

Maintenance and Cleaning of Vacuum Aspirator: 

You should follow the points below to keep the device fully functional and extend its lifespan.

  • It would help to avoid spraying the cleanser into the vacuum aspirator while cleaning the device. It will cause the probability of contaminating the device and the surface to increase.
  • You should unplug the device from the power source when cleaning as it will not only be safe for the experiment but will reduce the chance of the vacuum aspirator getting damaged, prolonging its lifetime.
  • You should wear the required protective industry-standard gloves for cleaning the vacuum aspirator.


The aspirator works by creating the differential pressure inside and outside the bottle, and due to the negative pressure, the liquid gets pulled inside the collection bottle and stored there. These vacuum aspirators are used for multiple multi-purposes, e.g. for the cell culture medium disposal, for the separation of liquids in the laboratory, and for the withdrawal of supernatant liquid so that the remaining fluids become pure and impurity-free. This is one of the most frequently used lab equipment to facilitate laboratory operations and productivity. 



Leave a Reply

Your email address will not be published. Required fields are marked *