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How to Remove Liquid Waste Using Vacuum Aspirator
January 27, 2024

How to Remove Liquid Waste Using Vacuum Aspirator

How to Remove Liquid Waste Using Vacuum Aspirator

Safely handling the liquid waste during the experiments is somewhat challenging via a vacuum aspirator. Using a vacuum aspirator very carefully is much more necessary to not mix the liquid waste with filtered liquescent. The separation process of waste liquid gets easy with vacuum aspiration. Most labs working on liquid waste recovery in biological, chemical, or even radioactive liquids principally used the vacuum pump for the liquid aspiration. However, the vacuum is a great replacement equipment for the vacuum pump, and it also works effectively and efficiently. This vacuum aspirator is mainly used in liquid separation, RNA/DNA extraction, microplate waste removal, and separating different liquids and solids from different mixtures. 

This liquid aspirator provides a convenient, easy-to-handle compact container with a safe liquid disposal system. This system is very easy to carry, suitable to clean, and use every time you pick. This is itself a complete unit, majorly assembled by multiple components. 

How to Use a Vacuum Aspirator? 

Following are the major steps you can follow to use this apparatus in the laboratory. 

  • Turn the power switch available at the backside of the aspirator equipment. 
  • Turn the vacuum control knob clockwise; now set the exact vacuum level, and the vacuum will increase gradually up to the desired mark. 
  • The light will become steady, and it will also indicate that the vacuum has reached the mark.
  • Now, on the hand operator, press the button to start the suction process in the liquid aspirator machine. 
  • Move the latch to the indicated position for persistent use. 

Disassembling of Vacuum Aspirator 

These are the few points that are necessary for the machine cleaning. 

  • First, take apart every component of the liquid aspirator apparatus. 
  • Now unplug the apparatus for the basic electrical source
  • Disconnect the tube from the cap, aspirator handle, and base unit
  • Disconnect level detection cable
  • Now turn the air release cap anti-clockwise so that the pressure of the equipment will release ultimately
  • Now remove the cap from the bottle

Cleaning of Vacuum Aspirator  

  • First, clean the housing with a lint-free cloth. 
  • Now soak this clothing piece into the soap solution or in 70% isopropanol or ethanol.
  • Never use bleach in the apparatus’s vacuum aspirator (sodium hypochlorite), as it can damage the equipment components. 
  • This is necessary to change the hydrophobic filter daily or at least every three months.
  • Always rinse the apparatus when using different solvents inside or any hazardous material, and rinse it with water or with a 70% ethanol solution. 
  • All parts in contact with the liquid must be autoclaved, comprising a waste collection bottle, cap with tube fittings, silicon tubing, and hand operator. 

Maintenance of the Vacuum Aspirator 

This apparatus doesn’t require any special service or maintenance, if the suction power gets reduced, then check the equipment inside, as it can be due to the blockage in the tube or filters attached to the pumps. This may be remedied by exchanging the tubes or by changing the filter parts. 

Applications of Vacuum Aspirators 

A vacuum aspirator in the laboratory can be used for various purposes, just as:

  • It can be used for the disposal of supernatant fluids from laboratory vessels.
  • The vacuum aspirator is also used for the extraction of RNA/DNA 
  • For the suction of metabolized media from the petri dish, this vacuum aspirator is an effective laboratory every day using apparatus.
  • This is one of the most effective equipment for microplate waste removal in the laboratory.
  • In addition, the vacuum aspirator apparatus is effective for the withdrawal of biological immunology reagents. 
  • Moreover, for separating variable liquids from solids and vice versa, this is the only efficient laboratory apparatus for fluid waste removal. 


The vacuum aspirator is one of the most demanding apparatus in the laboratory to separate different liquid fluids from one another and form solid samples. The aspirator uses vacuum pressure to suck the liquid and make the sample wastage free so that the user can easily perform laboratory functions and get accurate results at the end. However, these aspirators mainly include multiple components like the tube, cap, vacuum pump, and the main housing body. The cleaning of the vacuum aspirator is also necessary for the next time usage, but before cleaning, disassembling the apparatus is obligatory for the better clearance of remaining wastes inside the equipment. Thus, it can, later on, be rinsed with the isopropylidene or ethanol solution for better disinfection inside. 

This aspirator is used in the laboratory for various purposes for extracting RNA/DNA from any sample, for the suction of media from cell culture, or any tissue culture so that another medium can be poured into the petri dish for the next living cell cultivation. Other than this, supernatant liquid can only be withdrawn using vacuum aspirators in chemical, biological, and even radioactive laboratories. 

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